- Can culture beat Covid-19? Evidence that exposure to facemasks with cultural symbols increases solidarity -
Facemasks have become integral to everyday life. We propose that exposure to facemasks with a solidarity-related cultural symbol can activate cultural values such as mutual trust and increase corresponding interpersonal perceptions, thereby enhancing collective resilience in the Covid-19 pandemic. In three (two of which preregistered) studies, we examined whether exposure to facemasks with a solidarity-related cultural symbol predicts positive interpersonal perceptions, and whether this depends on death awareness. Across studies, exposure to facemasks with a cultural symbol (either pride flag or National Health Service) increased positive interpersonal perceptions, an index of solidarity, in people for whom this symbol represents a meaningful social identity. This was found whether participants were reminded of death, a neutral experience, or a negative experience. Importantly, in Study 3, exposure to facemasks with a solidarity-related cultural symbol (vs. surgical) led to greater increases in positive interpersonal perceptions when death awareness was high. Together, our findings suggest that wearing facemasks with a cultural symbol that relates to solidarity can be a vehicle for shaping people’s personality impressions of others. Applied directions for the activation of people’s social identities via facemask selection to promote collective resilience in the Covid-19 pandemic are discussed.
- COVID-19 on mind: Daily worry about the coronavirus is linked to negative affect experienced during mind-wandering but not during dreaming -
Despite a surge of studies on the effects of COVID-19 on our well-being, we know little about how the pandemic is reflected in people’s spontaneous thoughts and experiences, such as mind-wandering (or daydreaming) during wakefulness and dreaming during sleep. We investigated whether and how COVID-19 related general concern, anxiety, and daily worry are associated with the daily fluctuation of the affective quality of mind-wandering and dreaming, and to what extent these associations can be explained by poor sleep quality. We used ecological momentary assessment by asking participants to rate the affect they experienced during mind-wandering and dreaming in daily logs over a two-week period. Our preregistered analyses based on 1758 dream logs from 172 individuals and 1517 mind-wandering logs from 153 individuals showed that, on days when people experienced more worry, they reported higher levels of negative affect, and lower levels of positive affect, during mind-wandering. Only daily sleep quality was associated with affect experienced during dreaming: on nights with poorer sleep quality people reported experiencing more negative and less positive affect in dreams and were more likely to experience nightmares. Exploratory analyses demonstrated that: (a) COVID-19 related general concern, anxiety, and daily worry were not related to daily sleep quality; (b) individuals who experienced more negative affect during mind-wandering also experienced more negative affect during dreaming, and (c) negative affect during mind-wandering fully mediated the relationship between daily COVID-19 worry and dream affect at the between-person level. As such, daily COVID-19 worry is linked to the affective quality of mind-wandering but not to that of dreaming.
- Selection analysis identifies unusual clustered mutational changes in Omicron lineage BA.1 that likely impact Spike function -
Among the 30 non-synonymous nucleotide substitutions in the Omicron S-gene are 13 that have only rarely been seen in other SARS-CoV-2 sequences. These mutations cluster within three functionally important regions of the S-gene at sites that will likely impact (i) interactions between subunits of the Spike trimer and the predisposition of subunits to shift from down to up configurations, (ii) interactions of Spike with ACE2 receptors, and (iii) the priming of Spike for membrane fusion. We show here that, based on both the rarity of these 13 mutations in intrapatient sequencing reads and patterns of selection at the codon sites where the mutations occur in SARS-CoV-2 and related sarbecoviruses, prior to the emergence of Omicron the mutations would have been predicted to decrease the fitness of any virus within which they occurred. We further propose that the mutations in each of the three clusters therefore cooperatively interact to both mitigate their individual fitness costs, and, in combination with other mutations, adaptively alter the function of Spike. Given the evident epidemic growth advantages of Omicron over all previously known SARS-CoV-2 lineages, it is crucial to determine both how such complex and highly adaptive mutation constellations were assembled within the Omicron S-gene, and why, despite unprecedented global genomic surveillance efforts, the early stages of this assembly process went completely undetected.
- xia2.multiplex: a multi-crystal data analysis pipeline -
In macromolecular crystallography radiation damage limits the amount of data that can be collected from a single crystal. It is often necessary to merge data sets from multiple crystals, for example small-wedge data collections on micro-crystals, in situ room-temperature data collections, and collection from membrane proteins in lipidic mesophase. Whilst indexing and integration of individual data sets may be relatively straightforward with existing software, merging multiple data sets from small wedges presents new challenges. Identification of a consensus symmetry can be problematic, particularly in the presence of a potential indexing ambiguity. Furthermore, the presence of non- isomorphous or poor-quality data sets may reduce the overall quality of the final merged data set. To facilitate and help optimise the scaling and merging of multiple data sets, we developed a new program, xia2.multiplex, which takes data sets individually integrated with DIALS and performs symmetry analysis, scaling and merging of multi-crystal data sets. xia2.multiplex also performs analysis of various pathologies that typically affect multi-crystal data sets, including non-isomorphism, radiation damage and preferential orientation. After describing a number of use cases, we demonstrate the benefit of xia2.multiplex within a wider autoprocessing framework in facilitating a multi-crystal experiment collected as part of in situ room-temperature fragment screening experiments on the SARS-CoV-2 main protease.
- Evidence of increased Cathepsin B/L and decreased TMPRSS2 usage for cell entry by the SARS-CoV-2 Omicron variant -
The SARS-CoV-2 Omicron variant appears to exhibit altered cell tropism and entry properties compared to other variants. Here, analysing recent data, we found a strong positive correlation between the entry efficiency of the Omicron variant and the relative usage of the host protease Cathepsin B/L by the original SARS-CoV-2 strain for cell entry. We developed a mathematical model to quantify entry efficiency in in vitro assays and found that the Omicron variant displayed >4-fold improved efficiency in using Cathepsin B/L for entry in 293T-ACE2 cells compared to the original strain. The preferential usage of Cathepsin B/L over TMPRSS2 for entry may explain the altered cell tropism of the Omicron variant and have implications for our understanding of its infectivity and transmissibility as well as for interventions.
- Mucociliary Transport Deficiency and Disease Progression in Syrian Hamsters with SARS-CoV-2 Infection -
Substantial clinical evidence supports the notion that ciliary function in the airways plays an important role in COVID-19 pathogenesis. Although ciliary damage has been observed in both in vitro and in vivo models, consequent impaired mucociliary transport (MCT) remains unknown for the intact MCT apparatus from an in vivo model of disease. Using golden Syrian hamsters, a common animal model that recapitulates human COVID-19, we quantitatively followed the time course of physiological, virological, and pathological changes upon SARS-CoV-2 infection, as well as the deficiency of the MCT apparatus using micro-optical coherence tomography, a novel method to visualize and simultaneously quantitate multiple aspects of the functional microanatomy of intact airways. Corresponding to progressive weight loss up to 7 days post- infection (dpi), viral detection and histopathological analysis in both the trachea and lung revealed steadily descending infection from the upper airways, as the main target of viral invasion, to lower airways and parenchymal lung, which are likely injured through indirect mechanisms. SARS-CoV-2 infection caused a 67% decrease in MCT rate as early as 2 dpi, largely due to diminished motile ciliation coverage, but not airway surface liquid depth, periciliary liquid depth, or cilia beat frequency of residual motile cilia. Further analysis indicated that the fewer motile cilia combined with abnormal ciliary motion of residual cilia contributed to the delayed MCT. The time course of physiological, virological, and pathological progression suggest that functional deficits of the MCT apparatus predispose to COVID-19 pathogenesis by extending viral retention and may be a risk factor for secondary infection. As a consequence, therapies directed towards the MCT apparatus deserve further investigation as a treatment modality.
- Obesity associated with attenuated tissue immune cell responses in COVID-19 -
Obesity is common and associated with more severe COVID-19, proposed to be in part related to an adipokine-driven pro-inflammatory state. Here we analysed single cell transcriptomes from bronchiolar lavage in three adult cohorts, comparing obese (Ob, body mass index (BMI) >30m2) and non-obese (N-Ob, BMI <30m2). Surprisingly, we found that Ob subjects had attenuated lung immune/inflammatory responses in SARS-CoV-2 infection, with decreased expression of interferon (IFN), IFN{gamma} and tumour necrosis factor (TNF) alpha response gene signatures in almost all lung epithelial and immune cell subsets, and lower expression of IFNG and TNF in specific lung immune cells. Analysis of peripheral blood immune cells in an independent adult cohort showed a similar, but less marked, reduction in type I IFN and IFN{gamma} response genes, as well as decreased serum IFN in Ob patients with SARS-CoV-2. Nasal immune cells from Ob children with COVID-19 also showed reduced enrichment of IFN and IFN{gamma} response genes. Altogether, these findings show blunted tissue immune responses in Ob COVID-19 patients, with clinical implications.
- CRISPR screens for host factors critical for infection by SARS-CoV-2 variants of concern identify GATA6 as a central modulator of ACE2. -
The global spread of SARS-CoV-2 led to the most challenging pandemic in this century, posing major economic and health challenges worldwide. Revealing host genes essential for infection by multiple variants of SASR-CoV-2 can provide insights into the virus pathogenesis, and facilitates the development of novel broad-spectrum host-directed therapeutics. Here, employing genome scale CRISPR screens, we provide a comprehensive data-set of cellular factors that are exploited by WT-SARS-CoV-2 as well as two additional recently emerged variants of concerns (VOCs), Alpha and Beta. These screens identified known and novel host factors critical for SARS-CoV-2 infection, including various components belonging to the Clathrin-dependent transport pathway, ubiquitination and Heparan sulfate biogenesis. In addition, the host phosphatidylglycerol biosynthesis processes appeared to have major anti-viral functions. Comparative analysis of the different VOCs revealed the host factors KREMEN2 and SETDB1 as potential unique candidates required only to the Alpha variant, providing a possible explanation for the increased infectivity of this variant. Furthermore, the analysis identified GATA6, a zinc finger transcription factor, as an essential pro viral gene for all variants inspected. We revealed that GATA6 directly regulates ACE2 transcription and accordingly, is critical for SARS-CoV-2 cell entry. Analysis of clinical samples collected from SARS-CoV-2 infected individuals showed an elevated level of GATA6, indicating the important role GATA6 may be playing in COVID-19 pathogenesis. Finally, pharmacological inhibition of GATA6 resulted in down-modulation of ACE2 and consequently to inhibition of the viral infectivity. Overall, we show GATA6 represents a target for the development of anti-SARS-CoV-2 therapeutic strategies and reaffirm the value of the CRISPR loss-of-function screens in providing a list of potential new targets for therapeutic interventions.
- Short-term instantaneous prophylaxis and efficient treatment against SARS-CoV-2 in hACE2 mice conferred by an intranasal nanobody (Nb22) -
Current COVID-19 vaccines need to take at least one month to complete inoculation and then become effective. Around 51% global population are still not fully vaccinated. Instantaneous protection is an unmet need among those who are not fully vaccinated. In addition, breakthrough infections caused by SARS-CoV-2 are widely reported. All these highlight the unmet needing for short-term instantaneous prophylaxis (STIP) in the communities where SARS-CoV-2 is circulating. Previously, we reported nanobodies isolated from an alpaca immunized with the spike protein, exhibiting ultrahigh potency against SARS-CoV-2 and its variants. Herein, we found that Nb22, among our previously reported nanobodies, exhibited ultrapotent neutralization against Delta variant with an IC50 value of 0.41 ng/ml (5.13 pM). Furthermore, the crystal structural analysis revealed that the binding of Nb22 to WH01 and Delta RBDs both effectively blocked the binding of RBD to hACE2. Additionally, intranasal Nb22 exhibited protection against SARS-CoV-2 Delta variant in the post-exposure prophylaxis (PEP) and pre-exposure prophylaxis (PrEP). Of note, intranasal Nb22 also demonstrated high efficacy against SARS-CoV-2 Delta variant in STIP for seven days administered by single dose and exhibited long-lasting retention in the respiratory system for at least one month administered by four doses, providing a means of instantaneous short-term prophylaxis against SARS-CoV-2. Thus, ultrahigh potency, long-lasting retention in the respiratory system as well as stability at room-temperature make the intranasal or inhaled Nb22 to be a potential therapeutic or STIP agent against SARS-CoV-2.
- RASCL: Rapid Assessment Of SARS-CoV-2 Clades Through Molecular Sequence Analysis -
An important component of efforts to manage the ongoing COVID19 pandemic is the Rapid Assessment of how natural selection contributes to the emergence and proliferation of potentially dangerous SARS-CoV-2 lineages and CLades (RASCL). The RASCL pipeline enables continuous comparative phylogenetics-based selection analyses of rapidly growing clade-focused genome surveillance datasets, such as those produced following the initial detection of potentially dangerous variants. From such datasets RASCL automatically generates down-sampled codon alignments of individual genes/ORFs containing contextualizing background reference sequences, analyzes these with a battery of selection tests, and outputs results as both machine readable JSON files, and interactive notebook-based visualizations.
- Immunokinetic Model for COVID-19 Patients -
In this paper, we develop a fractional-order differential model for the dynamics of immune responses to SARS-CoV-2 viral load in one host. In the model, a fractional-order derivative is incorporated to represent the effects of temporal long-run memory on immune cells and tissues for any age group of patients. The population of cytotoxic T-cells (CD8+), natural killer (NK) cells and infected viruses are unknown in this model. Some interesting sufficient conditions that ensure the asymptotic stability of the steady states are obtained. This model indicates some complex phenomena in COVID-19 such as “immune exhaustion” and “Long COVID”. Sensitivity analysis is also investigated for model parameters to determine the parameters that are effective in determining of the long COVID duration, disease control and future treatment as well as vaccine design. The model is verified with clinical and experimental data of 5 patients with COVID-19.
- The Influence of Mass Media on Italian Web Users during COVID-19: an Infodemiological Analysis. -
Background: Alongside the COVID-19 pandemic, the world has had to face a growing infodemic, which has caused severe damage to economic and health systems and has often compromised the effectiveness of infection containment regulations. Although this has spread mainly through social media, there are numerous occasions in which the mass media have shared dangerous information, giving resonance to statements without a scientific basis. For these reasons, infoveillance and infodemiology methods are increasingly exploited to monitor online information traffic. The same tools have also been used to make epidemiological predictions. Among these, Google Trends - a service by GoogleTM that quantifies the web interest of users in the form of relative search volume - has often been adopted by the scientific community. Objective: The purpose of this paper is to use Google Trends to estimate the impact of Italian mass media on users’ web searches in order to understand the role of press and television channels in both the infodemic and the interest of Italian netizens on COVID-19. Methods: First, from January 2020 to March 2021, we collected the headlines containing specific COVID-19-related keywords published on PubMed, Google, the Ministry of Health, and the most read newspapers in Italy. These keywords were selected based on previous literature and the related queries of Google Trends. Second, we evaluated the percentage of infodemic terms on these platforms. Third, through Google Trends, we looked for correlations between newspaper headlines and Google searches related to COVID-19. We assessed the significance and intensity of changes in user web searches through Welch’s t-test and percentage differences or increases. We also highlighted the presence of trends via the Mann-Kendall test. Finally, we analyzed the web interest in infodemic content posted on YouTube. In particular, we counted the number of views of videos containing disinformation for each channel considered. Results: During the first COVID-19 wave, the Italian press preferred to draw on infodemic terms (from 1.6% to 6.3%) and moderately infodemic terms (from 88% to 94%), while scientific sources favored the correct names (from 65% to 88%). The correlational analysis showed that the press heavily influenced users in adopting the terms to identify the novel coronavirus (best average correlation = 0.91, P-value <.001). The use of scientific denominations by the press reached acceptable values only during the third wave (about 80% except for Rai and Mediaset). Web queries about COVID-19 symptoms also appeared to be influenced by the press (best average correlation = .92, P<.007). Furthermore, users have shown a pronounced web interest in YouTube videos of an infodemic nature. Finally, the press gave resonance to serious fake news on COVID-19 that caused pronounced spikes of interest from web users. Conclusions: Our results suggest that the Italian mass media have played a decisive role both in the spread of the infodemic and in addressing netizens’ web interest, thus favoring the adoption of terms unsuitable for identifying the novel coronavirus (COVID- 19 disease). Therefore, it is highly advisable that the directors of news channels and newspapers be more cautious and government dissemination agencies exert more control over such news.
- A New Infodemiological Approach Through Google Trends: Longitudinal Analysis of COVID-19 Scientific and Infodemic Names in Italy -
COVID-19 has been classified by the scientific community as the worst pandemic in human history. The damage caused by the new disease was direct (e.g., deaths) and indirect (e.g., closure of economic activities). Within the latter category, we find infodemic phenomena such as the adoption of generic and stigmatizing names used to identify COVID-19 and the related novel coronavirus 2019 variants. These monikers have fostered the spread of health disinformation and misinformation, and fomented racism and segregation towards the Chinese population. In this regard, we present a comprehensive infodemiological picture of Italy from the epidemic outbreak in December 2019 until September 2021. In particular, we propose a new procedure to examine in detail the web interest of users in scientific and infodemic monikers linked to the identification of COVID-19. To do this, we exploited the online tool Google Trends. Our findings reveal the widespread use of multiple COVID-19-related names not considered in the previous literature, as well as a persistent trend in the adoption of stigmatizing and misleading terms. Inappropriate names for cataloging novel coronavirus 2019 variants of concern have even been adopted by national health agencies. Furthermore, we also showed that early denominations influenced user behavior for a long time and were difficult to replace. For these reasons, we suggest that the assignments of scientific names to new diseases are more timely and advise against mass media and international health authorities using terms linked to the geographical origin of the novel coronavirus 2019 variants.
- The Impact of Cheering on Sports Performance: Comparison of Serie A Statistics Before and During COVID-19. -
Background: The role of cheering in home advantage in sports performance is the subject of much debate. No definitive scientific evidence has so far been found capable of highlighting or denying its relevance. As pandemic restrictive measures (COVID-19) have prevented crowds from entering stadiums, analysis of the past two football seasons may reveal important new details. Objective: This paper aims to compare the statistics of the last two football seasons in Italy with the previous six, trying to highlight any pronounced and statistically significant changes due to the absence of cheering in the stadiums. Methods: We compared the average percentages of points obtained in home matches from 2013 to 2019 with those in the timelapse 2019-2021. The same operation was performed with referee statistics, such as fouls, penalties, and cards awarded against home teams. To do this, we used measures such as Welch’s t-test and percentage increases. The distributive normality of the datasets was evaluated with the Shapiro-Wilk test and a graphical control. The lack of trend in the series was assessed with the Mann-Kendall test and Sen’s Slope. Finally, Pearson and Spearman’s correlations were searched between the percentages of points collected in home matches and total points, year by year, from 2013 to 2021. Results: The average percentage of points collected by teams in home matches dropped by almost 8% (Welch’s t-test = -4.3). Moreover, the negative correlations between home collected points and total points in 2013-2019 timelapse have drastically diminished during the last two seasons (average Welch’s t-test = 6.0, 95% CI: 5.4 – 6.4), approaching zero. Finally, penalties against home teams have increased by 30% (Welch’s t-test = 2.6), reaching figures closer to 50%. Conclusions: This research provides statistical evidence in favor of the relevance of home advantage in the top football league in Italy, such as Serie A. During the anti-COVID-19 restrictive measures - which prevented public access to the stadiums - a net reduction in the points collected by the teams in home matches was detected. In addition, the number of penalties awarded against home teams has increased significantly, approaching the ideal 50%. Since there are valid psychological reasons in the literature to support the crowd’s impact on sports and refereeing performance, it is plausible that our findings are causally related to the absence of cheering. Finally, as the averages of points collected at home remained far from 50%, our results suggest that a not negligible part of the home advantage in Serie A is linked to factors independent of the audience. Future research can deepen the above phenomena from a theoretical-psychological point of view.
- The influence of mass media on Italian web users during COVID-19: an infodemiological analysis. -
Alongside the COVID-19 pandemic, the world has had to face a growing infodemic, which has caused severe damage to economic and health systems and has often compromised the effectiveness of infection containment regulations. Although this has spread mainly through social media, there are numerous occasions in which the mass media have shared dangerous information, giving resonance to statements without a scientific basis. For these reasons, infoveillance and infodemiology methods are increasingly exploited to monitor online information traffic. However, these tools have also been used to make epidemiological predictions. In particular, the “Google Trends” tool by GoogleTM has often been adopted by the scientific community to carry out this type of analysis. In this regard, the purpose of this paper is to estimate the impact of Italian mass media on users’ web searches in order to understand the role of the media in the infodemic and in the interest of Italian web users towards COVID-19. In particular, our results suggest that the Italian mass media have played a decisive role both in the spread of the infodemic and in addressing netizens’ web interest, favoring the adoption of terms unsuitable for identifying the novel coronavirus (COVID- 19 disease). Therefore, we suggest greater caution and attention by the directors of news channels and newspapers and greater control of the latter by government dissemination agencies.
Quantifying Viral Load in Respiratory Particles That Are Generated by Children and Adults With COVID-19 Infection - Condition: COVID-19
Intervention: Device: COVID-19 Aerosol Collection
Sponsor:
Massachusetts General Hospital
Recruiting
Evaluation of Safety & Efficacy of MIR 19 ® Inhalation Solution in Patients With Moderate COVID-19 - Condition: COVID-19
Interventions: Drug: MIR 19 ®; Combination Product: Standard COVID-19 therapy
Sponsors: National Research Center - Institute of Immunology Federal Medical-Biological Agency of Russia; St. Petersburg Research Institute of Vaccines and Sera
Completed
Plasma Exchange in Covid-19 Patients With Anti-interferon Autoantibodies - Condition: COVID-19
Intervention: Drug: Therapeutic plasma exchange
Sponsor:
Centre Hospitalier St Anne
Recruiting
Randomized Multicenter Study on the Efficacy and Safety of Favipiravir for Parenteral Administration Compared to Standard of Care in Hospitalized Patients With COVID-19 - Condition: COVID-19
Interventions: Drug: Favipiravir; Drug: Remdesivir
Sponsors: Promomed, LLC; Solyur Pharmaceuticals Group
Completed
Inhaled Heparin for Hospitalised Patients With Coronavirus Disease 2019 (COVID-19) - Condition: COVID-19
Intervention: Drug: unfractionated Heparin
Sponsors:
Australian National University; The George Institute; St George Hospital, Australia; St Vincent’s Hospital Melbourne; John Hunter Hospital; Royal North Shore Hospital
Not yet recruiting
Immunogenicity and Safety Study of a SCB-2019 Vaccine Booster Dose to Adults Who Previously Received Primary Series of Selected COVID-19 Vaccines - Condition: COVID-19
Intervention: Biological: Candidate vaccine, SCB-2019
Sponsor: Clover Biopharmaceuticals AUS Pty Ltd
Not yet recruiting
COVID-19 Messaging for Vaccination - Conditions: Vaccination Refusal; COVID-19 Pandemic
Interventions: Behavioral: Doctor Videos; Behavioral: Sharing Videos; Behavioral: Sharing Videos (Influencers); Behavioral: Vaccine Ambassador; Behavioral: Video framing; Behavioral: Video order
Sponsors: Massachusetts Institute of Technology; Facebook, Inc.; Code3; Stanford University; Harvard University; Yale University; Johns Hopkins University; Massachusetts General Hospital; Ludwig-Maximilians - University of Munich; National Institutes of Health (NIH)
Recruiting
Respiratory Physiotherapy and Neurorehabilitation in Patients With Post-covid19 Sequelae. - Condition: COVID-19 Pandemic
Intervention: Other: respiratory treatment
Sponsor: Universidad Católica de Ávila
Not yet recruiting
IMPACT OF THERAPEUTIC PLASMA EXCHANGE ON ACQUIRED VACCINAL ANTI-SARS-CoV-2 ANTIBODIES. - Condition: COVID-19
Intervention: Diagnostic Test: Evolution of antibodies titre
Sponsor: Cliniques universitaires Saint-Luc- Université Catholique de Louvain
Recruiting
Spa Rehabilitation, Antioxidant and Bioenergetic Supportive Treatment of Patients With Post-Covid-19 Syndrome - Condition: COVID-19 Respiratory Infection
Interventions: Dietary Supplement: ubiquinol (reduced coenzyme Q10); Other: mountain spa rehabilitation; Diagnostic Test: 2x14 ml of peripheral blood collected in a tube with anticoagulant
Sponsors: Comenius University; Sanatórium of Dr. Guhr, n.o.
Completed
The Effect of Dietary Intervation on Endothelial Glycocalyx in COVID-19 Patients. - Conditions: COVID-19; Endothelial Dysfunction
Interventions:
Dietary Supplement: Food supplement Endocalyx; Dietary Supplement: Placebo
Sponsor:
University of Athens
Not yet recruiting
Public Support for COVID-19 Test Allocation - Conditions: Health Equity; COVID-19
Interventions: Behavioral: First Come, First Served; Behavioral: Random; Behavioral: Disadvantaged Priority & Random
Sponsor: University of Pennsylvania
Completed
Randomized Study of Efficacy of Different Treatment Regimens of Olokizumab - Condition: COVID-19
Interventions: Drug: Olokizumab; Drug: Standard therapy
Sponsors: R-Pharm; Federal Budget Institution of Science “Central Research Institute of Epidemiology” of the Rospotrebnadzor; Group of companies Medsi, JSС
Recruiting
Study to Assess the Safety, Tolerability and Explore the Immunogenicity of EG-COVID in Healthy Adult Volunteers - Condition: COVID-19 Vaccine
Interventions: Drug: EG-COVID-003; Drug: EG-COVID-001
Sponsors: EyeGene Inc.; Novotech (Australia) Pty Limited
Not yet recruiting
Evaluation of IGM-6268 in Healthy Adults and Patients With Mild to Moderate COVID-19 - Conditions: Healthy Volunteers; COVID-19
Interventions: Drug: IGM-6268; Drug: Placebo
Sponsor: IGM Biosciences, Inc.
Not yet recruiting
Development and utilization of a surrogate SARS-CoV-2 viral neutralization assay to assess mRNA vaccine responses - CONCLUSIONS: A competitive ELISA based on inhibition of RBD-ACE2r attachment correlates well with PRNT, quantifies significantly higher activity among vaccine recipients with prior COVID (vs. those without), and highlights marked declines in surrogate neutralizing activity over a 6 month period post vaccination. The findings raise concern about the duration of vaccine responses and potential need for booster shots.
RBD conjugate vaccine with a built-in TLR1/2 agonist is highly immunogenic against SARS-CoV-2 and variants of concern - The coronavirus 2019 (COVID-19) pandemic is causing serious impacts in the world, and safe and effective vaccines and medicines are the best methods to combat the disease. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein plays a key role in interacting with the angiotensin-converting enzyme 2 (ACE2) receptor, and is regarded as an important target of vaccines. Herein, we constructed the adjuvant-protein conjugate Pam(3)CSK(4)-RBD as a vaccine candidate, in which the N-terminal…
Neutralizing SARS-CoV-2 by dimeric side chain-to-side chain cross-linked ACE2 peptide mimetics - We present the finding of a dimeric ACE2 peptide mimetic designed through side chain cross-linking and covalent dimerization. It has a binding affinity of 16 nM for the SARS-CoV-2 spike RBD, and effectively inhibits the SARS-CoV-2 pseudovirus in Huh7-hACE2 cells with an IC(50) of 190 nM and neutralizes the authentic SARS-CoV-2 in Caco2 cells with an IC(50) of 2.4 μM. Our study should provide a new insight for the optimization of peptide-based anti-SARS-CoV-2 inhibitors.
Antiinflammatory potential of nano-curcumin as an alternative therapeutic agent for the treatment of mild-to- moderate hospitalized COVID-19 patients in a placebo-controlled clinical trial - The present study conducted a placebo-controlled clinical trial to evaluate the impact of nano-curcumin on the inflammatory cytokines in mild-to-moderate hospitalized COVID-19 patients. A total of 60 COVID-19 patients were randomly divided into nano-curcumin and control groups, and then they received 240 mg/day nano-curcumin for 7 days. The clinical manifestation and laboratory parameters in patients were recorded on days 0 and seven. Also, SYBR Green real-time PCR and ELISA techniques were…
Metadichol: A Novel Nanolipid Formulation That Inhibits SARS-CoV-2 and a Multitude of Pathological Viruses In Vitro - Increasing outbreaks of new pathogenic viruses have promoted the exploration of novel alternatives to time-consuming vaccines. Thus, it is necessary to develop a universal approach to halt the spread of new and unknown viruses as they are discovered. One such promising approach is to target lipid membranes, which are common to all viruses and bacteria. The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has reaffirmed the importance of interactions between the virus…
Factors associated with insomnia among frontline nurses during COVID-19: a cross-sectional survey study - CONCLUSION: The present study’s findings help to explain how pandemic consequences can be associated with insomnia. Additionally, the findings make a significant contribution to better understanding the role of neuroticism, emotion dysregulation, beliefs, and psychopathology vulnerability in the development of insomnia among nurses. The findings suggest the potential influence of cognitive behavioral therapy for insomnia (CBT-I) and transdiagnostic integrated therapies that could be incorporated…
Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis - Enterovirus infections can cause severe complications, such as poliomyelitis, encephalitis, myocarditis, meningitis, neurological pulmonary edema, and even death. Here, we used genome-wide CRISPR screens to gain new insight into the mechanism by which enteroviruses co-opt host pathways to potentiate replication and propagation. We found that acyl- coenzyme A synthetase long-chain family member 4 (ACSL4) is involved in viral replication organelle formation. ACSL4 is a key component of ferroptosis,…
JIB-04 Has Broad-Spectrum Antiviral Activity and Inhibits SARS-CoV-2 Replication and Coronavirus Pathogenesis - Pathogenic coronaviruses are a major threat to global public health. Here, using a recombinant reporter virus-based compound screening approach, we identified small-molecule inhibitors that potently block the replication of severe acute respiratory syndrome virus 2 (SARS-CoV-2). Among them, JIB-04 inhibited SARS-CoV-2 replication in Vero E6 cells with a 50% effective concentration of 695 nM, with a specificity index of greater than 1,000. JIB-04 showed in vitro antiviral activity in multiple…
Expression and immunogenicity of nsp10 protein of porcine epidemic diarrhea virus - Porcine epidemic diarrhea virus (PEDV), a swine enteropathogenic coronavirus, causes lethal watery diarrhea to the piglets, which poses significant economic losses and public health concerns. The nsp10 protein of PEDV is essential regulatory subunits that are critical for virus replication. Since PEDV nsp10 is a crucial regulator of viral RNA synthesis, it is promising that nsp10 might become anti-virus drugs target or candidate for rapid diagnosis of PEDV infection. In this study, the PEDV…
ACE2 internalization induced by a SARS-CoV-2 recombinant protein is modulated by angiotensin II type 1 and bradykinin 2 receptors - AIMS: Angiotensin-converting enzyme 2 (ACE2) is a key regulator of the renin-angiotensin system (RAS) recently identified as the membrane receptor for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here we aim to study whether two receptors from RAS, the angiotensin receptor type 1 (AT1R) and the bradykinin 2 receptor (B2R) modulate ACE2 internalization induced by a recombinant receptor binding domain (RBD) of SARS-CoV-2 spike protein. Also, we investigated the impact of ACE2…
Evaluation of adult patients with atopic dermatitis treated with dupilumab: a single-center real-life experience - Adult atopic dermatitis (AD), especially adult-onset type appears to have different clinical manifestations. Dupilumab is an IL-4 receptor antagonist used in patients with moderate and severe atopic dermatitis, aged 12 years and older and it works by inhibiting the IL-4 and IL-13 signaling pathway. The purpose of our study is to retrospectively investigate the side effect profile and drug efficacy of thirteen adult patients who received dupilumab treatment and to evaluate the drug use status and…
Molecular modelling and structure-activity relationship of a natural derivative of o-hydroxybenzoate as a potent inhibitor of dual NSP3 and NSP12 of SARS-CoV-2: in silico study - The nsp3 macrodomain and nsp12 (RdRp) enzymes are strongly implicated in the virulent regulation of the host immune response and viral replication of SARS-CoV-2, making them plausible therapeutic targets for mitigating infectivity. Remdesivir remains the only FDA-approved small-molecule inhibitor of the nsp12 in clinical conditions while none has been approved yet for the nsp3 macrodomain. In this study, 69,067 natural compounds from the IBScreen database were screened for efficacious potentials…
Characterization of the SARS-CoV-2 ExoN (nsp14ExoN-nsp10) complex: implications for its role in viral genome stability and inhibitor identification - The SARS-CoV-2 coronavirus is the causal agent of the current global pandemic. SARS-CoV-2 belongs to an order, Nidovirales, with very large RNA genomes. It is proposed that the fidelity of coronavirus (CoV) genome replication is aided by an RNA nuclease complex, comprising the non-structural proteins 14 and 10 (nsp14-nsp10), an attractive target for antiviral inhibition. Our results validate reports that the SARS-CoV-2 nsp14-nsp10 complex has RNase activity. Detailed functional characterization…
I(2)-Catalyzed Carbonylation of alpha-Methylene Ketones to Synthesize 1,2-Diaryl Diketones and Antiviral Quinoxalines in One Pot - An efficient approach for the synthesis of 1,2-diaryl diketones was developed from readily available α-methylene ketones by catalysis of I(2). In the same oxidation system, a novel one-pot procedure was established for the construction of antiviral and anticancer quinoxalines. The reactions proceeded well with a wide variety of substrates and good functional group tolerance, affording desired compounds in moderate to excellent yields. Quinoxalines 4ca and 4ad inhibited viral entry of SARS-CoV-2…
Inhibition of SARS-CoV-2 infection in human iPSC-derived cardiomyocytes by targeting the Sigma-1 receptor disrupts cytoarchitecture and beating - SARS-CoV-2 infects cardiac cells and causes heart dysfunction. Conditions such as myocarditis and arrhythmia have been reported in COVID-19 patients. The Sigma-1 receptor (S1R) is a ubiquitously expressed chaperone that plays a central role in cardiomyocyte function. S1R has been proposed as a therapeutic target because it may affect SARS-CoV-2 replication; however, the impact of the inhibition of S1R in human cardiomyocytes remains to be described. In this study, we investigated the…
IDENTIFICATION AND ALARM SYSTEM FOR FACIAL CORONA MASK USING CNN BASED IMAGE PROCESSING - tThe covid-19 epidemic is the world’s largest wake-up call for people to pay attention to their own and society’s health. One thing to keep in mind is that there is a segment of the population that has been exposed to the covid-19 virus and has generated antibodies without developing any significant illnesses and is continuing to be healthy. This indicates that a significant section of the population, even excluding the elderly, lacks the necessary bodily immunity to combat a Viral infection. As terrible as covid-19 is on a global scale, developing personal health standards and preventative measures for any pathogenic virus as a community would have spared many lives. In’this work, a camera is combined with an image processing system to recognise facial masks, which may be improved in a variety of ways. First and foremost, this method is meant to identify masks on a single person’s face. While this method is efficient in identifying someone has a mask, it does not ensure that they will wear it all of the time. The most effective update for this task is to install a camera with a wide field of view so that many individuals can be seen in the frame, and the faces of those who aren’t wearing markings can be identified, as well as the number of people and the timing. - link
ANTIMICROBIAL SANITIZING FORMULATION - An antimicrobial sanitizing formulation, comprising, i) isopropyl alcohol in the range of 0.1%- 80% w/w, ii) an emollient in the range of 0.1%-15% w/w, iii) hydrogen peroxide in the range of 0.1 0.13% w/w, iv) citric acid in the range of 0.1% to 2.0% w/w, v) silver nitrate in the range of 0.1% to 0.5% w/w, and vi) a fragrance imparting agent in the range of 0.1% to 2.0% w/w. - link
A HEALTH BAND WITH A BIOMETRIC MODULE AND WORKING METHOD THEREOF - The present invention discloses a health band with a biometric module and method thereof. The assembly includes, but not limited to, a plurality of sensors configured to gather health data associated with a predefined symptom of a medical condition of a user; a memory unit configured to store the data and an interface, which is configured to determine the medical condition using the data;a processing unit configured to execute the application; and a notification facility configured to provide a notification upon receiving from the interface an instruction associated with the notification, wherein the notification is associated with a drug reminder and the like. - link
RNA 검출 방법 - 본 발명은 RNA의 분석 및 검출 방법에 관한 것이다. 특히, 본 발명은 특히, 본 발명은 짧은 염기서열의 RNA까지 분석이 가능하면서도 높은 민감도 및 정확도로 정량적 검출까지 가능하여 감염증, 암 등 여러 질환의 진단 용도로도 널리 활용될 수 있다. - link
REUNION OF PHOTOTHERMAL THERAPY WITH MXENE ADSORBED UREMIC TOXINS AND CYTOKINES: A SHILED FOR COVID-19 PATENTS - The COVID-19 pandemic has created havoc throughout the world. The disease has proved to be more fatalfor patients having comorbidities like diabetics, lungs and kidney infections, etc. In the case of COVID-19 patientsI having kidney injury, the. removal of uremic toxins from the blood is hindered and there is a rapid surge in the levelj of cytokine hormone resulting in the death of the patient in a short interval of time. To resolve this issue,iI; researchers have examined that the immediate removal of these toxins can improve the condition of the patient to a |greater extent. Studies have also found the presence of SARS CoV-2 viral RNAs in the blood of COVID-19patients, which risks their life as well as impacts the blood transfusion process, especially in the case ofasymptomatic patients. Hence it is required to control the surge of cytokines and uremic toxins as well as disinfectthe blood of the patient from the virus. MXenes, having a foam-like porous structure and hydrophilic negativesurface functionalization have greater adsorption efficiency as well as superior photothermal activity. Utilizingthese properties of MXenes, the MXene membranes can be used in the dialyzer that can help in the efficient andBiuick removal of the uremic toxins, cytokines, and other impurities from the blood. Along with this the greaterTJAdsorption efficiency of MXenes to amino acids result in the trapping of the SARS CoV-2 viruses on the surface J)3>f the MXene. Many researchers as well as the WHO have proved the efficient reduction of the viral copy numbersjjvith the increase of temperature. Hence, followed by the trapping of the viruses, the implementation of"Zphotothermal Therapy can result in the inactivation and denaturation of the viruses and their respective viral RNAsBJlby the produced heat. The same process can be repeated several times to get better results. This whole process canr>oQ-esult in impurity-free and infection-free blood, that can be returned back to the body of the patient or can be!— I Sitilized for the blood transfusion process without any risk of infection.IM - link
REDUCING AND STOPPING OXYGEN WASTAGE IN HOSPITAL - In an aspect, the present invention discloses a system (200) for prevention and reduction of oxygen wastage from oxygen mask (202). The system (200) includes the oxygen mask (202) having straps; a tension sensor (204), the tension sensor being sensitive towards tension produced in the straps as the oxygen gets leakage through sides of the mask (202); a processor configured in alignment with the tension sensor (204); and a buzzer (206) in alignment with processor. - link
编码SARS-COV-2病毒C.37突变株抗原的DNA分子、DNA疫苗及应用 - 本发明涉及生物技术领域，具体而言，提供了一种编码SARS‑COV‑2病毒C.37突变株抗原的DNA分子、DNA疫苗及应用。本发明提供的SEQ ID NO：1核酸序列在真核表达系统中能够高效转录和表达，而且具有免疫原性，表现在体液免疫和细胞免疫应答中，以此作为活性成分的核酸疫苗同样具有良好的免疫原性。 - link
SARS-COV-2病毒B.1.617.2突变株DNA疫苗及应用 - 本发明涉及生物技术领域，具体而言，提供了一种编码SARS‑COV‑2病毒B.1.617.2突变株抗原的DNA分子、DNA疫苗及应用。本发明提供的SEQ ID NO：1核酸序列在真核表达系统中能够高效转录和表达，而且具有免疫原性，表现在体液免疫和细胞免疫应答中，以此作为活性成分的核酸疫苗同样具有良好的免疫原性。 - link
Hung Thanh Phan COVID-19 NEW SOLUTION - - link
A METHOD TO REVEAL MOTIF PATTERNS OF COVID-19 USING MULTIPLE SEQUENCE ALIGNMENT - This present invention consists of different levels of computation and work in a pipeline manner i.e., input of one will be output of another and it is sequential process. Input data given in form of nucleotide sequence (DNA) of different COVID-19 patients (1). Using these nucleotide sequence perform mutation if possible and arrange them in a sequential order (2). Arrange number of nucleotide sequences of different patients in row wise and also compute number of characters in each row. (3). Compute frequency of occurrence of character in column wise and create a matrix having 4 rows and maximum sequence length will be the column size (4). Find the character like A, T, C, and G which one has maximum score and similarly find for each column to produce a final sequence (5). - link